Volume 1, Issue 1 (6-2015)
J Health Res Commun 2015, 1(1): 35-41 |
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Shahryari A, Nikaeen M, Hassnzadeh A. Assessment of Clostridium perfringens in determination of fecal pollution of drinking water in comparion to indicators bacteria. J Health Res Commun 2015; 1 (1) :35-41
URL: http://jhc.mazums.ac.ir/article-1-35-en.html
URL: http://jhc.mazums.ac.ir/article-1-35-en.html
Assistant Professor, Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: (12096 Views)
Introduction & Purpose: Fecal coliforms and Escherichia coli have been conventionally used as indicators of microbial pollution of drinking water. The European Directive on drinking water quality has recently included Clostridium perfringens as one of the microbiological organisms suitable for using in control of the quality of water for human consumption. This study endeavored to assess the efficacy of Clostridium perfringens in comparison with fecal coliforms and Escherichia coli in detection of the microbial pollution of water sources.
Methods: In this study, 60 raw water samples were collected from drinking water in the Isfahan province, Iran from May to November 2012. Fecal coliforms, Escherichia coli, fecal streptococci and Clostridium perfringens were investigated by multiple-tube fermentation technique (MFT) using double-strength medium in 10 series of tubes according to the standard methods. Statistical analyses were carried out using SPSS version 18 at the 95% confidence level (α = 0.05).
Results: The results of this study showed that 46.2 % of water samples were positive with regard to fecal contamination at least for one bacterial indicator. Clostridium perfringens was the most frequently detected indicator (36.5%), followed by fecal streptococci (34.6%), fecal coliforms (28.8%) and Escherichia coli (25%). According to Pearson’s coefficient, the most significant correlation was found between Clostridium perfringens and fecal streptococci (0.88).
Conclusion: Clostridium perfringens is an important indicator for water-quality monitoring in communities, where raw water is used directly for human consumption without purification. Evaluation of the spores of this bacterium can provide an added margin of safety for predicting the microbial quality of drinking water.
Methods: In this study, 60 raw water samples were collected from drinking water in the Isfahan province, Iran from May to November 2012. Fecal coliforms, Escherichia coli, fecal streptococci and Clostridium perfringens were investigated by multiple-tube fermentation technique (MFT) using double-strength medium in 10 series of tubes according to the standard methods. Statistical analyses were carried out using SPSS version 18 at the 95% confidence level (α = 0.05).
Results: The results of this study showed that 46.2 % of water samples were positive with regard to fecal contamination at least for one bacterial indicator. Clostridium perfringens was the most frequently detected indicator (36.5%), followed by fecal streptococci (34.6%), fecal coliforms (28.8%) and Escherichia coli (25%). According to Pearson’s coefficient, the most significant correlation was found between Clostridium perfringens and fecal streptococci (0.88).
Conclusion: Clostridium perfringens is an important indicator for water-quality monitoring in communities, where raw water is used directly for human consumption without purification. Evaluation of the spores of this bacterium can provide an added margin of safety for predicting the microbial quality of drinking water.
Keywords: Water pollution, Fecal streptococci, Escherichia coli, fecal indicator bacteria, Clostridium perfringens
Type of Study: Research(Original) |
Subject:
میکروبیولوژی
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